Cellworks > Angiogenesis Assays > HUVECs and Fibroblasts - Build a Bespoke Angiogenesis Assay

Build a Bespoke Angiogenesis Assay

'Cellworks Angiogenesis tested Human Umbilical Vein Endothelial Cells (HUVEC) and Human Dermal Fibroblasts (HDF) in combination produce tubules.'

Cells, medium packages and staining kits are available separately, allowing total flexibility in angiogenesis assay design.

This option provides complete control over the number and cell types present, and the opportunity to manipulate the cells prior to culturing, for example by transfection.

Benefits

  • Purchasing components separately allows total flexibility in assay design
  • Control over the number of cells used
  • Additional cell types can be added
  • Transfect to manipulate cells prior to culturing
  • Cells have proven angiogenesis potential (tested in angiogenesis assay)
  • Tubule growth analysed with Cellworks AngioSys software

Please click here to download the protocol for a bespoke angiogenesis assay.

Angiogenesis Visualisation of tubule formation through GFP transfection of HUVEC cells
Angiogenesis Fibroblasts
Angiogenesis Tubules stained with anti CD31 (PECAM-1)

Human Umbilical Vein Endothelial Cells (HUVEC) pooled donor - Angiogenesis Tested

   Product Code: ZHC-2102
  • Produce tubules in conjunction with Cellworks Angiogenesis Tested Human Dermal Fibroblasts (ZHC-5102), when cultured with Cellworks Angiogenesis Seeding and Growth Medium (ZHA-1960 and ZHA-1970).
  • Validated for use in the manufacture of Cellworks V2a Kits (Product Code: ZHA-4000).
  • Minimum of 500,000 cryopreserved cells.
  • Guaranteed for 10 population doublings.

Human Dermal Fibroblasts (HDF) - adult - Angiogenesis Tested

   Product Code: ZHC-5102
  • Produce tubules in conjunction with Cellworks Angiogenesis tested Human Umbilical Vein Endothelial Cells (ZHC-2102), with Cellworks Angiogenesis Seeding Medium and Angiogenesis Growth Medium (ZHA-1960 and ZHA-1970).
  • Validated for use in the manufacture of Cellworks V2a Kits (Product Code: ZHA-4000).
  • Minimum of 1,000,000 cryopreserved cells.
  • Guaranteed for 10 population doublings.

Early Passage Human Umbilical Vein Endothelial Cells (HUVEC) - pooled donor

   Product Code: ZHC-2301
  • Early Passage HUVECs are cryopreserved at the end of the secondary culture.
  • A cost effective alternative to buying primary cells.
  • Minimum of 500,000 cells.
  • Guaranteed for 10 population doublings.

Supporting Reagents

CD31 Tubule Staining Kit, Product Code: ZHA-1225

Angiogenesis Assay Control Kit, Product Code: ZHA-1300

Media and Supplements

Angiogenesis Growth Medium Package Validated for use with the V2a™ Kit, Product Code: ZHA-1970

Angiogenesis Seeding Medium Package Validated for use with the V2a™ Kit, Product Code: ZHA-1960

Human Large Vessel Endothelial Cell Growth Medium Package, Product Code: ZHM-2953

Human Fibroblast Growth Medium Package, Product Code: ZHM-5923

Quantification of In Vitro Angiogenesis through Image Analysis

Cellworks offer two possibilities for analysis of in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts. Both provide accurate quantification of the stimulatory and inhibitory effects of different agents and output the lengths of the tubule complexes as well as the numbers of junctions in each.

If you require a pre formed angiogenesis assay kit, rather than bespoke options, please visit the V2a Kit Vasculogenesis to Angiogenesis page.

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