Cellworks > GFP V2a Kit™ - Vasculogenesis to Angiogenesis

GFP V2a Kit

GFP V2a Kit™ - Vasculogenesis to Angiogenesis

Product Code ZHA-4100

The GFP V2a Kit™ - Vasculogenesis to Angiogenesis, is a validated, in vitro, human primary cell co -culture model of angiogenesis that allows the dynamic measurement of all phases of the angiogenic process.

Tubules formed contain a lumen and resemble the morphology and range of capillary lengths found in microvascular beds in vivo.

The GFP V2a Kit™ enables the dynamic measurement of vascular tube formation.

  • It is a kinetic, quantitative, 24 well co culture angiogenesis model ,utilising lentivirally infected human umbilical vein endothelial cells (HUVEC) expressing GFP co-cultured with normal human dermal fibroblasts (HDF).
  • This co-culture model recapitulates all phases of the in vivo process from vasculogenesis to angiogenesis (including: cell activation, cell proliferation, migration, elongation and anastomosis)
  • Test compounds, media or tissue explants can be added at any point in the pathway - from the onset of vasculogenesis through to advanced angiogenesis - giving total assay flexibility
  • The GFP- V2a Kit includes: +/- controls, media, supplements and a 24 well plate
  • V2a Co-Culture Cells are provided frozen allowing long term storage and user determined assay initiation
  • Tubule growth is quantifiable using AngioSys 2.0 Software
Tubule Formation in response to VEGF

Tubule Formation in response to VEGF

Untreated Tubules

Untreated Tubules

Tubule Formation in response to Suramin

Tubule Formation in response to Suramin

GFP V2a Kit™ - Vasculogenesis to Angiogenesis

Product Code: ZHA-4100

Kit Contents:

  • Ampoule of cryopreserved V2a Co-Culture Cells - GFP HUVEC
  • 24 well tissue culture plate
  • 25ml V2a Seeding Medium
  • 125ml V2a Growth Medium
  • V2a Seeding Medium Supplement
  • V2a Growth Medium Supplement
  • Angiogenesis Control compounds (VEGF and Suramin)

For storage conditions and additional equipment required please read the protocol. Download the protocol here.

Please click here to view a series of YouTube videos which will assist you with the V2a Kit.

Molecules shown to be active in the Cellworks V2a Kit™

Inhibitors

VEGF neutralising antibody, VEGF antisense oligonucleotide, Collagen IV neutralising antibody, PECAM-1 neutralising antibody, VEGF-R antagonists, Thrombospondin derivatives, TNF alpha, TGF b1, aVb3 integrin antagonists, Suramin, Curcumin, Fumagillin, TNP-740 (fumagillin analogue), Colchicine, Cytochalasin B, Erucic acid, Ginsengosides, RGD peptide, Endostatin, Thymine, Thalidomide and analogues, PAI-1 inhibitors MMP inhibitors, MGBG (methylglyoxalbisguanylhydrozone), some conditioned media.

Enhancers

VEGF, PIDGF, BFGF, 2-deoxyribose-1-P, Most tumour cell conditioned media, Wound fluid, Ascitic fluid from leiomyosarcoma patient, Ascorbic acid (biphasic response).

Note: that this represents only the molecules whose identity we know. We have tested many proprietary compounds from various laboratories that were active in both senses but which were supplied as coded samples whose structure is not known to us.

Quantification of In Vitro Angiogenesis through Image Analysis

Cellworks offer two possibilities for analysis of in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts. Both provide accurate quantification of the stimulatory and inhibitory effects of different agents and output the lengths of the tubule complexes as well as the numbers of junctions in each.

If you require further flexibility to your angiogenesis assay, please visit our Build a Bespoke Angiogenesis Assay page.

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