Cellworks > Angiogenesis Service > Angiogenesis Complete Assay Service

Angiogenesis Complete Assay Service

Analyse the Angiogenic Pathway: Cellworks Angiogenesis Service.

Compounds, media and tissue explants can be tested at any point in the angiogenic pathway using the Cellworks Angiogenesis Service. The service includes the following stages and can be tailored to your needs:

  1. Performing Angiogenesis Assays
  2. Capture and Analysis of Images
  3. Provision of Raw Data
  4. Bespoke Options

1) Performing Angiogenesis Assays

Using the Cellworks V2a Kit - Vasculogenesis to Angiogensis, Cellworks specialists offer customers the ability to outsource angiogenesis experiments.

The V2a Kit - Vasculogenesis to Angiogensis model is an in vitro human primary cell co-culture model of angiogenesis that includes all of the phases of the angiogenic process. Tubules formed contain a lumen and resemble the morphology and range of capillary lengths found in microvascular beds in vivo.

Test compounds, media or tissue explants can be added at any point in the pathway - from the onset of vasculogenesis continuing through to advanced angiogenesis (including: cell activation, cell proliferation, migration, elongation and anastomosis), allowing intervention in the cellular growth process at any stage. The Cellworks Angiogenesis Service can be carried out on any number of wells required by the customer, from a single well to multiple assays over several plates.

2) Capture and Analysis of Images

Tubule Formation in response to VEGF
Tubule Formation in response to VEGF

Untreated Tubules
Untreated Tubules

Tubule Formation in response to Suramin
Tubule Formation in response to Suramin

Angiogenesis experiments fixed and stained with anti-CD31 antibody are photographed and analysed using the Angiosys 2.0 software; a custom built software for measurement of tubule growth. Take advantage of the expertise of the in house specialists, with years of experience performing and analysing these assays at Cellworks.

3) Provision of Raw Data

Reports are presented in an excel format and contain raw data including details of the number of tubules, the number of junctions, the total tubule length, and the mean tubule length for each assay.

Report Example:

angiosys screenshot

4) Bespoke Options

The service is tailored to each project and all parts can be adjusted to your individual requirements. For further information about the service please contact cellworks@caltagmedsystems.co.uk  or call +44 (0)1280 827460.

To view the Cellworks range of reagents for in-house angiogenesis assays, please select from the links below:

The following manuals are also available:

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