Cellworks > Angiogenesis Assays > V2a Kit

V2a Kit

V2a Kit™ - Vasculogenesis to Angiogenesis

'An in vitro human primary cell co-culture model of angiogenesis that includes all the phases of the angiogenic process'

Tubules formed contain a lumen and resemble the morphology and range of capillary lengths found in microvascular beds in vivo.

Benefits

  • Analyse the growth pathway from vasculogenesis to angiogenesis (including: cell activation, cell proliferation, migration, elongation and anastomosis)
  • Add test compounds, media or tissue explants at any point in the pathway - from the onset of vasculogenesis continuing through to advanced angiogenesis
  • Intervene in the cellular growth process at any stage
  • Kit includes: +/- controls, media, supplements, staining components, plate
  • Frozen cells: perform assay at your convenience
  • Large batches of cells in stock: minimal batch to batch variation
  • Tubule growth is quantifiable using AngioSys 2.0 Software

Tubule Formation in response to VEGF
Tubule Formation in response to VEGF

Untreated Tubules
Untreated Tubules

Tubule Formation in response to Suramin
Tubule Formation in response to Suramin

V2a Kit™ - Vasculogenesis to Angiogenesis

   Product Code: ZHA-4000

Kit Contents:

  • Ampoule of cryopreserved V2a Cells
  • 24 well tissue culture plate
  • 25ml V2a Seeding Medium
  • 125ml V2a Growth Medium
  • V2a Seeding Medium Supplement
  • V2a Growth Medium Supplement
  • Angiogenesis Control compounds (VEGF and Suramin)
  • Mouse anti-human CD31 Primary Antibody
  • Goat anti-mouse IgG Alkaline Phosphatase Secondary Antibody
  • BCIP/NBT insoluble substrate (for permanent record of tubule staining)

For storage conditions and additional equipment required please read the protocol. Download the protocol here.

Molecules shown to be active in the Cellworks V2a Kit™:

Inhibitors

VEGF neutralising antibody, VEGF antisense oligonucleotide, Collagen IV neutralising antibody, PECAM-1 neutralising antibody, VEGF-R antagonists, Thrombospondin derivatives, TNF alpha, TGF b1, aVb3 integrin antagonists, Suramin, Curcumin, Fumagillin, TNP-740 (fumagillin analogue), Colchicine, Cytochalasin B, Erucic acid, Ginsengosides, RGD peptide, Decorin, Endostatin, Thymine, Thalidomide and analogues, PAI-1 inhibitors MMP inhibitors, MGBG (methylglyoxalbis(guanylhydrozone), some conditioned media.

Enhancers

VEGF, PIDGF, BFGF, 2-deoxyribose-1-P, Most tumour cell conditioned media, Wound fluid, Ascitic fluid from leiomyosarcoma patient, Ascorbic acid (biphasic response).

Note: that this represents only the molecules whose identity we know. We have tested many proprietary compounds from various laboratories that were active in both senses but which were supplied as coded samples whose structure is not known to us.

Quantification of In Vitro Angiogenesis through Image Analysis

Cellworks offer two possibilities for analysis of in vitro angiogenesis assays based on the co-culture of endothelial cells with fibroblasts. Both provide accurate quantification of the stimulatory and inhibitory effects of different agents and output the lengths of the tubule complexes as well as the numbers of junctions in each.

If you require further flexibility to your angiogenesis assay, please visit our Build a Bespoke Angiogenesis Assay page.

AngioKit

Some publications make reference to the "AngioKit", a live cell assay previously offered. This has been superseded by the V2a Kit - Vasculogenesis to Angiogenesis due to customer feedback.

The V2a Kit Vasculogenesis to Angiogenesis is an enhancement to the AngioKit as:

  • It is easy to ship
  • It provides increased flexibility in assay parameters
  • Earlier stages of Vasculogenesis can be studied
  • All cells can be purchased from the same batch
  • The level of intervention is improved

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